Journal Article

A Possible Role of Glutathione and Glutathione Disulfide in Tracheary Element Differentiation in the Cultured Mesophyll Cells of <i>Zinnia elegans</i>

Kenji Henmi, Seiji Tsuboi, Taku Demura, Hiroo Fukuda, Masaki Iwabuchi and Ken’ichi Ogawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 6, pages 673-676
Published in print June 2001 | ISSN: 0032-0781
Published online June 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce072
A Possible Role of Glutathione and Glutathione Disulfide in Tracheary Element Differentiation in the Cultured Mesophyll Cells of Zinnia elegans

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We investigated the relationship between the cellular redox state of GSH or GSSG and tracheary element (TE) differentiation using a Zinnia experimental system, in which isolated mesophyll cells transdifferentiate to TEs. TE differentiation was suppressed by the application of l-buthionine sulfoximine (BSO), a potent inhibitor of GSH biosynthesis, at the early stage of cell culture. Application of GSSG at the early culture stage promoted the differentiation, but that of GSH or GSSG at an advanced period of culture suppressed the differentiation. Application of GSH and GSSG nullified the TE differentiation-suppressing effect of BSO. The results suggest that changes in the redox states of GSH and GSSG have a role in TE differentiation.

Keywords: Key words: l-Buthionine sulfoximine — Glutathione — γ-Glutamylcysteine syntethase (EC 6.3.2.2) — Redox — Tracheary element differentiation — Zinnia elegans.; Abbreviations: BSO, l-buthionine sulfoximine; γ-ECS, γ-glutamylcysteine syntethase; TEs, tracheary elements.

Journal Article.  2297 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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