Journal Article

Functional Analysis of <i>psbV</i> and a Novel c-type Cytochrome Gene <i>psbV2</i> of the Thermophilic Cyanobacterium <i>Thermosynechococcus</i> <i>elongatus</i> Strain BP-1

Hiroshi Katoh, Suwako Itoh, Jian-Ren Shen and Masahiko Ikeuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 6, pages 599-607
Published in print June 2001 | ISSN: 0032-0781
Published online June 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce074
Functional Analysis of psbV and a Novel c-type Cytochrome Gene psbV2 of the Thermophilic Cyanobacterium Thermosynechococcus elongatus Strain BP-1

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Cytochrome c-550 is an extrinsic protein associated with photosystem II (PSII) in cyanobacteria and lower eukaryotic algae and plays an important role in the water-splitting reaction. The gene (psbV) for cytochrome c-550 was cloned from the thermophilic cyanobacteria Thermosynechococcus (formerly Synechococcus) elongatus and T. (formerly Synechococcus) vulcanus. In both genomes, located downstream of psbV were a novel gene (designated psbV2) for a c-type cytochrome and petJ for cytochrome c-553. The deduced product of psbV2 showed composite similarities to psbV and petJ. Phenotype of psbV-disruptant in Thermosynechococcus was practically the same as that reported in Synechocystis sp. PCC 6803. Either psbV or psbV2 gene of T. elongatus was expressed in the psbV-disruptant of Synechocystis sp. PCC 6803, which resulted in recovery of the photoautotrophic growth. However, the enhanced requirement of Ca2+ or Cl ions in the psbV-disruptant of Synechocystis was suppressed by expression of psbV but not by expression of psbV2. Thus, it is concluded that psbV2 can partly replace the role of psbV in PSII. The close tandem arrangement of psbV/psbV2/petJ implies that psbV2 was created by gene duplication and intergenic recombination during evolution.

Keywords: Key words: Cytochrome — Photosystem II — psbV — psbV2 — Thermophilic cyanobacterium — Thermosynechococcus (Synechococcus) elongatus.; Abbreviations: 2,6DCBQ, 2,6-dichloro-p-benzoquinone; 2,6DMBQ, 2,6-dimethyl-p-bezoquinone.

Journal Article.  5834 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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