Journal Article

RNA Editing of the Ribosomal Protein S13 Transcripts in Magnolia and Sunflower Mitochondria

Teresa Maria R. Regina, Loredana Lopez, Rita Bruno and Carla Quagliariello

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 7, pages 768-774
Published in print July 2001 | ISSN: 0032-0781
Published online July 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce088
RNA Editing of the Ribosomal Protein S13 Transcripts in Magnolia and Sunflower Mitochondria

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  • Biochemistry
  • Molecular and Cell Biology
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To obtain information on ribosomal protein gene content and RNA editing in plant mitochondria, we investigated the conservation of the gene for ribosomal protein S13 (rps13) in the mitochondrial genomes of Magnolia spp. and Helianthus annuus. A complete rps13 open reading frame was identified in the mitochondrial genome of Magnolia and Helianthus. Comparison of genomic and cDNA sequences revealed that transcripts from the mitochondrial rps13 locus in both Magnolia and Helianthus undergo RNA editing suggesting the functionality of the related gene. Six and four editing events in the rps13 transcript population of Magnolia and Helianthus mitochondria, respectively, cause critical changes of codons and, consequently, induce 5 and 3.4% amino acid modifications in the respective genomically-encoded S13 polypeptides. Both editing pattern and efficiency of RNA editing differ in the reverse transcription (RT)-PCR-derived cDNA populations from the rps13 locus from the analyzed plant species with only the Magnolia rps13 being fully edited.

Keywords: Key words: Helianthus annuus — Magnolia spp. — Plant mitochondria — Ribosomal protein — RNA editing — rps13.; Abbreviations: ATPase, ATP synthase; BSA, bovine serum albumine; MOPS, 3-[N-Morpholino] propanesulfonic acid; mtRNA, mitochondrial RNA; ORF, open reading frame; rps13, gene for ribosomal protein S13; r-protein(s), ribosomal protein(s); RT-PCR, reverse transcription-PCR; PVP, polyvinyl-pyrrolidone.

Journal Article.  5040 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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