Journal Article

Involvement of Calcium Ion in the Stimulated Shoot Elongation of Arrowhead Tubers under Anaerobic Conditions

Shinsuke Tamura, Hitoshi Kuramochi and Kimiharu Ishizawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 7, pages 717-722
Published in print July 2001 | ISSN: 0032-0781
Published online July 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce090
Involvement of Calcium Ion in the Stimulated Shoot Elongation of Arrowhead Tubers under Anaerobic Conditions

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Shoot elongation of arrowhead (Sagittaria pygmaea Miq.) tubers was stimulated in anaerobic conditions. The anaerobic elongation was attributed to stimulation of cell elongation in the middle of the shoots. The anaerobic elongation of the shoots was severely inhibited by ethylene glycol bis(β-aminoethylether)-N,N,N′,N-tetraacetic acid (EGTA). The EGTA inhibition was completely nullified by exogenous CaCl2, which acts as an enhancer of anaerobic elongation. Moreover, calcium channel blockers, varapamil, diltiazem and LaCl3, inhibited the anaerobic elongation enhanced by CaCl2. These results showed that calcium plays an important role in stimulating the elongation in anaerobic conditions. Incorporation of 45Ca into the shoot tissues was measured to determine the involvement of calcium uptake in anaerobic elongation. Incorporation of 45Ca into the cell sap, which was collected from frozen and thawed shoots after thorough washing with LaCl3, was significantly stimulated in anaerobic conditions. Verapamil and diltiazem prevented the stimulation of 45Ca incorporation in anaerobic conditions. These results suggest that calcium uptake from the medium serves to enhance shoot elongation of arrowhead tubers under anaerobic conditions.

Keywords: Key words: Anaerobiosis — Anoxia — Aquatic plants — Calcium — Elongation — Sagittaria pygmaea.; Abbreviation: EGTA, ethylene glycol bis(β-aminoethylether)-N,N,N′,N′-tetraacetic acid.

Journal Article.  4344 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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