Journal Article

Visualization of an FtsZ Ring in Chloroplasts of <i>Lilium longiflorum</i> Leaves

Toshiyuki Mori, Haruko Kuroiwa, Manabu Takahara, Shin-ya Miyagishima and Tsuneyoshi Kuroiwa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 6, pages 555-559
Published in print June 2001 | ISSN: 0032-0781
Published online June 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce095
Visualization of an FtsZ Ring in Chloroplasts of Lilium longiflorum Leaves

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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FtsZ is a bacterial division protein which forms a ring at the leading edge of the cell division site. To date, a hypothesis that the plant FtsZ forms the same structure in chloroplast division is proposed, but has not been demonstrated yet. In this study, recombinant LlFtsZ (Lilium longiflorum FtsZ) protein was produced from a previously isolated ftsZ cDNA clone [Mori and Tanaka (2000) Protoplasma 214: 57] and used to raise polyclonal anti-LlFtsZ antibodies in rabbits. In immunoblot analysis with the total protein extracted from L. longiflorum leaves, purified antibodies specifically recognized LlFtsZ whose molecular mass was approximately 43 kDa. This size corresponded to that of the recombinant LlFtsZ protein lacking N-terminal sequence, which suggests that the full-length LlFtsZ translation product has a putative N-terminal signal peptide. Moreover, fluorescent and electron microscopy revealed that the anti-LlFtsZ antibodies recognized ring structures at stromal side of the constriction point of dividing chloroplasts. Here, we show direct evidence that FtsZ ring is involved in chloroplast division.

Keywords: Key words: Bacterial division — Chloroplast division — FtsZ ring — Lilium longiflorum.; Abbreviations: FITC, fluorescein isothiocyanate; IPTG, isopropyl β-d-thiogalactopyranoside; PBS, phosphate-buffered saline; PD ring, plastid-dividing ring; PVDF, polyvinylidene difluoride; TBS, Tris-buffered saline.

Journal Article.  3121 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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