Journal Article

<i>Azospirillum</i> spp. metabolize[ 17,17-<sup>2</sup>H<sub>2</sub>]gibberellin A<sub>20</sub> to[ 17,17-<sup>2</sup>H<sub>2</sub>]gibberellin A<sub>1</sub> in vivo in <i>dy</i> rice mutant seedlings

Fabricio D. Cassán, Carlos D. Lucangeli, Rubén Bottini and Patricia N. Piccoli

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 7, pages 763-767
Published in print July 2001 | ISSN: 0032-0781
Published online July 2001 | e-ISSN: 1471-9053 | DOI:
Azospirillum spp. metabolize[
17,17-2H2]gibberellin A20 to[
17,17-2H2]gibberellin A1 in
vivo in dy rice mutant seedlings

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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Azospirillum spp. are endophytic bacteria with beneficial effects on cereals — effects partially attributed to gibberellin production by the microorganisms. Azospirillum lipoferum and Azospirillum brasilense inoculated to rice dy mutant reversed dwarfism in seedlings incubated with [17,17-2H2]GA20 with formation of [17,17-2H2]GA1, showing the in vivo capacity to perform the 3β-hydroxylation. When Prohexadione-Ca, an inhibitor of late steps in gibberellin biosynthesis, was added to the culture medium, no complementation was observed and no [17,17-2H2]GA1 was produced. The latter suggests that the bacterial operating enzyme may be a 2-oxoglutarate-dependent dioxygenase, similar to those of plants.

Keywords: Key words: Azospirillum spp. — Dwarf Rice (Oryza sativa L.) dy mutant — Gibberellin 3β-hydroxylation.; Abbreviations: CFU, colony forming units; Me-TMSi, methyl ester-trimethylsilyl ether; NFb, nitrogen free biotin-based medium; OD540, optical density at 540 nm.

Journal Article.  3589 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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