Journal Article

Partial Characterization of Farnesyl and Geranylgeranyl Diphosphatases Induced in Rice Seedlings by UV-C Irradiation

Jaehyuk Nah, Seon-Joo Song and Kyoungwhan Back

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 8, pages 864-867
Published in print August 2001 | ISSN: 0032-0781
Published online August 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce102
Partial Characterization of Farnesyl and Geranylgeranyl Diphosphatases Induced in Rice Seedlings by UV-C Irradiation

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This study reports the identification and characterization of allyl diphosphatases (EC 3.1.7.1) in plants by using rice seedlings. Two distinct Mg2+-independent allyl diphosphatases, which convert farnesyl diphosphate (FDP) and geranylgeranyl diphosphate (GGDP) into farnesol and geranylgeraniol, respectively, were induced in rice seedlings irradiated with UV-C. Farnesyl diphosphatase (FDPase) and geranylgeranyl diphosphatase (GGDPase) are located in the microsomal fraction. The relative specific activity of FDPase was much higher than the specific activity of GGDPase. FDPase activity was inhibited by GGDP (50% inhibition at 5 µM) and geranyl diphosphate (50% inhibition at 100 µM). In contrast, GGDPase activity was inhibited 50% by 50 µM isopentenyl diphosphate or 100 µM FDP. The optimal pH for FDPase was 6.3 and for GGDPase was 7.9.

Keywords: Key words: Allyl diphosphatase — Farnesol — Farnesyl diphosphatase — Geranylgeraniol — Geranylgeranyl diphosphatase — UV-C — Rice seedling.; Abbreviations: FDP, farnesyl diphosphate; FDPase, farnesyl diphosphatase; GGDPase, geranylgeranyl diphosphatase; GPP, geranyl diphosphate; IPP, isopentenyl diphosphate; IPTG, isopropyl β-d-thiogalactopyranoside; GAP, glyceraldehydes 3-phosphate; NP, ρ-nitrophenyl phosphate.

Journal Article.  2534 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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