Journal Article

Localization of CYP86B1 in the Outer Envelope of Chloroplasts

Christy J. W. Watson, John E. Froehlich, Caroline A. Josefsson, Clint Chapple, Francis Durst, Irene Benveniste and Ronald C. Coolbaugh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 8, pages 873-878
Published in print August 2001 | ISSN: 0032-0781
Published online August 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce110
Localization of CYP86B1 in the Outer Envelope of Chloroplasts

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  • Biochemistry
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CYP86B1 was cloned from a cDNA library and the protein expressed in E. coli. The protein gave the expected carbon monoxide difference spectrum. Using in vitro import assays with isolated pea chloroplasts, CYP86B1 was shown to be associated with the outer chloroplastic envelope membrane. This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase.

Keywords: Key words: Chloroplast localization — CYP86B1 — Monooxygenase — P450.; Abbreviations: AOS, allene oxide synthase; C4H, cinnamic acid 4-hydroxylase; HPL, hydroperoxide lyase; CHAPS, 3-[(-Cholamidopropyl) dimethylammonio]-1-propanesulfonate, CYP, cytochrome P450; ER, endoplasmic reticulum; OM14, chloroplast outer membrane marker protein.

Journal Article.  4681 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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