Journal Article

Possible Involvement of Auxin-Induced Ethylene in an Apoptotic Cell Death during Temperature-Sensitive Lethality Expressed by Hybrid between <i>Nicotiana glutinosa</i> and <i>N. repanda</i>

Tetsuya Yamada, Wataru Marubashi, Teruko Nakamura and Masaru Niwa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 9, pages 923-930
Published in print September 2001 | ISSN: 0032-0781
Published online September 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce114
Possible Involvement of Auxin-Induced Ethylene in an Apoptotic Cell Death during Temperature-Sensitive Lethality Expressed by Hybrid between Nicotiana glutinosa and N. repanda

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Interspecific hybrids of Nicotiana glutinosa L. × N. repanda Willd. express temperature-sensitive lethality induced by apoptotic cell death. Hybrid seedlings cultured at 28°C began to exhibit lethal symptoms during early growth stages, and then they showed a high level of endogenous auxin compared with those of parental seedlings. Meanwhile, the level of auxin in hybrid seedlings cultured at 32°C, which is a condition suppressing the lethality of this cross combination, was equal to or lower than those of parental seedlings. Administration of 2,3,5-triiodobenzoic acid (TIBA) as an auxin transport inhibitor into the hybrid seedlings suppressed lethal symptoms and had a life-extending effect. Additionally, TIBA has an effect to suppress DNA fragmentation, which is one of characteristics of apoptosis and has been detected in the hybrid seedlings expressing the lethality. Administration of aminooxyacetic acid (AOA) as an ethylene synthesis inhibitor, which could inhibit ethylene production, also showed the same effects as TIBA for the lethality. From these results, we suggested that auxin and ethylene were involved in an apoptotic cell death during the lethality, and the abnormal increase of endogenous auxin may lead to the ethylene production in hybrid seedlings during early growth stages.

Keywords: Key words: Apoptotic cell death — Auxin-induced ethylene — DNA fragmentation — Interspecific hybrid — Temperature-sensitive lethality — Tobacco.; Abbreviations: 2,4,6-T; 2,4,6-trichlorophenoxyacetic acid, AOA, aminooxy acetic acid; ACC, 1-amino-cyclopropane-1-carboxylic acid; HPLC, high performance liquid chromatography; IAA, indole acetic acid; IPA, indole pyruvic acid; PCIB, 4-chlorophenoxyisobutyric acid; SAM, S-adenosylmethionine; TIBA, 2,3,5-triiodobenzoic acid.

Journal Article.  5798 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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