Journal Article

Identification of <i>Lhcb</i> Gene Family Encoding the Light-harvesting Chlorophyll-<i>a/b</i> Proteins of Photosystem II in <i>Chlamydomonas reinhardtii</i>

Haruhiko Teramoto, Taka-aki Ono and Jun Minagawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 8, pages 849-856
Published in print August 2001 | ISSN: 0032-0781
Published online August 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce115
Identification of Lhcb Gene Family Encoding the Light-harvesting Chlorophyll-a/b Proteins of Photosystem II in Chlamydomonas reinhardtii

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The Lhcb gene family in green plants encodes several light-harvesting Chl a/b-binding (LHC) proteins that collect and transfer light energy to the reaction centers of PSII. We comprehensively characterized the Lhcb gene family in the unicellular green alga, Chlamydomonas reinhardtii, using the expressed sequence tag (EST) databases. A total of 699 among over 15,000 ESTs related to the Lhcb genes were assigned to eight, including four new, genes that we isolated and sequenced here. A sequence comparison revealed that six of the Lhcb genes from C. reinhardtii correspond to the major LHC (LHCII) proteins from higher plants, and that the other two genes (Lhcb4 and Lhcb5) correspond to the minor LHC proteins (CP29 and CP26). No ESTs corresponding to another minor LHC protein (CP24) were found. The six LHCII proteins in C. reinhardtii cannot be assigned to any of the three types proposed for higher plants (Lhcb1–Lhcb3), but were classified as follows: Type I is encoded by LhcII-1.1, LhcII-1.2 and LhcII-1.3, and Types II, III and IV are encoded by LhcII-2, LhcII-3 and LhcII-4, respectively. These findings suggest that the ancestral LHC protein diverged into LHCII, CP29 and CP26 before, and that LHCII diverged into multiple types after the phylogenetic separation of green algae and higher plants.

Keywords: Key words: Chlamydomonas reinhardtii — Light-harvesting chlorophyll-a/b protein — Photosystem II.; Abbreviations: DCCD, dicyclohexylcarbodiimide; EST, expressed sequence tag; LHC, light-harvesting chlorophyll a/b-binding; NPQ, non-photochemical quenching.

Journal Article.  6224 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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