Journal Article

Mastoparan Alters Subcellular Distribution of Profilin and Remodels F-Actin Cytoskeleton in Cells of Maize Root Apices

František Baluška, Matthias von Witsch, Mechthild Peters, Andrej Hlavačka and Dieter Volkmann

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 9, pages 912-922
Published in print September 2001 | ISSN: 0032-0781
Published online September 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce116
Mastoparan Alters Subcellular Distribution of Profilin and
Remodels F-Actin Cytoskeleton in Cells of Maize Root
Apices

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Indirect immunofluorescence localization of profilin in cells of maize root apices revealed that this abundant protein was present both in the cytoplasm and within nuclei. Nucleo-cytoplasmic partitioning of profilin exhibits tissue-specific and developmental features. Mastoparan-mediated activation of heterotrimeric G-proteins, presumably through triggering a phosphoinositide-signaling pathway based on phosphatidylinositol-4,5-bisphosphate (PIP2), induced relocalization of profilin from nuclei into the cytoplasm of root apex cells. In contrast, PIP2 accumulated within nuclei of mastoparan-treated root cells. Intriguingly, cytoplasmic accumulation of profilin was associated with remodeling of F-actin arrays in root apex cells. Specifically, dense F-actin networks were dismantled and distinct actin patches became associated with the periphery of small vacuoles. On the other hand, disruption of F-actin with the G-actin sequestering agent latrunculin B does not affect the subcellular distribution of profilin or PIP2. These data suggest that nuclear profilin can mediate a stimulus–response action on the actin cytoskeleton which is somehow linked to a phosphoinositide-signaling cascade.

Keywords: Key words: Actin — Maize root — Nucleus — Phosphoinositide signaling — Profilin.; Abbreviations: AFs, Actin filaments; PA, Phosphatidic acid; PI, Phosphoinositide; PIP2, Phosphatidylinositol-4,5-bisphosphate; PLP, Poly-l-proline; PRA, Profilin rabbit antibody; ZmPRO3, Zea mays profilin isoform 3.

Journal Article.  7591 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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