Journal Article

Redox Changes Accompanying the Degradation of Seed Storage Proteins in Germinating Rice

Hiroyuki Yano, Joshua H. Wong, Myeong-Je Cho and Bob B. Buchanan

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 8, pages 879-883
Published in print August 2001 | ISSN: 0032-0781
Published online August 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce119
Redox Changes Accompanying the Degradation of Seed Storage
Proteins in Germinating
Rice

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The mobilization of storage proteins (glutelins) in germinating rice seeds was accompanied by an ordered sequential combination of proteolysis and reduction of disulfide groups. Mobilization was followed by application of non-reducing/reducing two dimensional-PAGE after monobromobimane labeling of the sulfhydryl groups of the proteins in intact seeds.

Keywords: Key words: 2D-PAGE— Disulfide bond reduction — Monobromobimane — Protein mobilization — Rice glutelin — Thioredoxin.; Abbreviations: mBBr, monobromobimane; 2D, two-dimensional.

Journal Article.  2438 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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