Journal Article

Oxidative Stress Activates ATMPK6, an Arabidopsis Homologue of MAP Kinase

Takashi Yuasa, Kazuya Ichimura, Tsuyoshi Mizoguchi and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 9, pages 1012-1016
Published in print September 2001 | ISSN: 0032-0781
Published online September 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce123
Oxidative Stress Activates ATMPK6, an Arabidopsis Homologue of MAP
Kinase

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Mitogen-activated protein kinase (MAPK) cascades function in biotic and abiotic stress responses in plants. We analysed effect of oxidative stress on the activation of ATMPK6, an Arabidopsis thaliana MAPK, in Arabidopsis T87 cultured cells and rosette leaves using anti-ATMPK6 specific antibody. ATMPK6 in T87 cells was strongly activated by reactive oxygen species (ROS) such as H2O2 and KO2. In leaves, ATMPK6 was activated by paraquat and 3-amino-1,2,4-triazole (a catalase inhibitor). These results indicate that ATMPK6 is one of the candidates for signal mediators in response to abiotic or biotic sources for ROS in Arabidopsis.

Keywords: Key words: Arabidopsis — MAP kinase — Oxidative stress — Phosphorylation.; Abbreviations: 3-AT, 3-amino-1,2,4-triazole; DTT, dithiothreitol; ERK, extracellular-regulated protein kinase; JA, jasmonic acid; MAPK, mitogen-activated protein kinase; MBP, myelin basic protein; PMSF, phenylmethylsulfonyl fluoride; ROS, reactive oxygen species; SA, salicylic acid; SIPK, salicylic-acid-induced protein kinase; TMV, tobacco mosaic virus; WIPK, wound-induced protein kinase.

Journal Article.  3921 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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