Journal Article

Progress of Lignification Mediated by Intercellular Transportation of Monolignols During Tracheary Element Differentiation of Isolated <i>Zinnia</i> Mesophyll Cells

Mami Hosokawa, Shiro Suzuki, Toshiaki Umezawa and Yasushi Sato

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 9, pages 959-968
Published in print September 2001 | ISSN: 0032-0781
Published online September 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce124
Progress of Lignification Mediated by Intercellular Transportation of Monolignols During Tracheary Element Differentiation of Isolated Zinnia Mesophyll Cells

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Tracheary element (TE) differentiation is a typical example of programmed cell death (PCD) in higher plants, and maturation of TEs is completed by degradation of all cell contents. However, lignification of TEs progresses even after PCD. We investigated how and whence monolignols are supplied to TEs which have undergone PCD during differentiation of isolated Zinnia mesophyll cells into TEs. Higher densities of cell culture induced greater lignification of TEs. Whereas the continuous exchanging of culture medium suppressed lignification of TEs, further addition of coniferyl alcohol into the exchanging medium reduced the suppression of lignification. Analysis of the culture medium by HPLC and GC-MS showed that coniferyl alcohol, coniferaldehyde, and sinapyl alcohol accumulated in TE inductive culture. The concentration of coniferyl alcohol peaked at the beginning of secondary wall thickening, decreased rapidly during secondary wall thickening, then increased again. These results indicated that lignification on TEs progresses by supply of monolignols from not only TEs themselves but also surrounding xylem parenchyma-like cells through medium in vitro.

Keywords: Key words: Coniferyl alcohol — Intercellular interaction — Lignification — Monolignol — Tracheary element — Zinnia elegans.; Abbreviations: AOPP, α-aminooxy-β-phenylpropionic acid; BA, benzyladenine; CA, coniferyl alcohol; CAD, cinnamyl alcohol dehydrogenase; CD, coniferaldehyde; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate:CoA ligase; DMSO, dimethylsulfoxide; GC-MS, gas chromatography-mass spectrometry; GUS, β-glucuronidase; NAA, 1-naphthaleneacetic acid; PAL, phenylalanine ammonia-lyase; PCD, programmed cell death; SA, sinapyl alcohol; SD, sinapaldehyde; TE, tracheary element.

Journal Article.  5786 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.