Journal Article

An Arabidopsis SNF1-Related Protein Kinase, AtSR1, Interacts with a Calcium-Binding Protein, AtCBL2, of Which Transcripts Respond to Light

Akira Nozawa, Nozomu Koizumi and Hiroshi Sano

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 9, pages 976-981
Published in print September 2001 | ISSN: 0032-0781
Published online September 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce126
An Arabidopsis SNF1-Related Protein Kinase, AtSR1, Interacts with a Calcium-Binding Protein, AtCBL2, of Which Transcripts Respond to Light

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  • Biochemistry
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AtSR1 is a protein kinase of Arabidopsis thaliana, which belongs to the SNF1-related protein kinase subfamily 3. We previously showed accumulation of its transcripts to be responsive to light. In this study, we examined the interaction between AtSR1 and six calcineurin B like proteins of Arabidopsis and found that AtSR1 prominently interacts with one of them, AtCBL2, by yeast two-hybrid assay. Interaction between AtSR1 and AtCBL2 could also be directly confirmed in vitro by pull down assay. RNA blot and reverse transcription-polymerase chain reaction analyses showed that transcripts of AtCBL2, and also of AtCBL1, another CBL, increased upon illumination of leaves. The physiological meaning of the interaction of AtSR1and AtCBL2 is not clear, but they presumably function in signal transduction of light.

Keywords: Key words: Arabidopsis thaliana — Calcineurin B-like protein — SNF1-related protein kinase.; Abbreviations: 3-AT, 3-aminotriazole; CBB, Coomassie Brilliant Blue; CBL, calcineurin B like protein; RT-PCR, reverse transcription-polymerase chain reaction; SC, synthetic complete; SnRK, SNF1-related protein kinase.

Journal Article.  4223 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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