Journal Article

The <i>Purple leaf</i> (<i>Pl</i>) Locus of Rice: the <i>Pl</i><i><sup>w</sup></i> Allele has a Complex Organization and Includes Two Genes Encoding Basic Helix-Loop-Helix Proteins Involved in Anthocyanin Biosynthesis

Wataru Sakamoto, Taku Ohmori, Keisuke Kageyama, Chikara Miyazaki, Akira Saito, Minoru Murata, Kazuhiko Noda and Masahiko Maekawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 9, pages 982-991
Published in print September 2001 | ISSN: 0032-0781
Published online September 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce128
The Purple leaf (Pl) Locus of Rice: the
Plw Allele has a Complex Organization and
Includes Two Genes Encoding Basic Helix-Loop-Helix Proteins Involved in
Anthocyanin
Biosynthesis

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The Purple leaf (Pl) locus of rice (Oryza sativa L.) affects regulation of anthocyanin biosynthesis in various plant tissues. The tissue-specific patterns of anthocyanin pigmentation, together with the syntenic relationship, indicate that the rice Pl locus may play a role in the anthocyanin pathway similar to the maize R/B loci. We isolated two cDNAs showing significant identity to the basic helix-loop-helix (bHLH) proteins found in the maize R gene family. OSB1 appeared to be allelic to the previously isolated R homologue, Ra1, but showed a striking difference at the C-terminus because of a 2-bp deletion. Characterization of the corresponding genomic region revealed that the sequence identical to a 5′-portion of OSB2 existed ~10-kb downstream of the OSB1 coding region. OSB2 lacks a conserved C-terminal domain. Restriction fragment length polymorphism analyses using an F2 population indicate that both genes co-segregate with the purple leaf phenotype. A transient complementation assay showed that the anthocyanin pathway is inducible by OSB1 or OSB2. These results suggest that the Plw allele may be complex and composed of at least two genes encoding bHLH proteins.

Keywords: Key words: Anthocyanin biosynthetic pathway — Basic-helix-loop-helix protein — Oryza sativa – Purple leaf.; Abbreviations: bHLH, basic helix loop helix; LA-PCR, long and accurate PCR; NLS, nuclear localization signal; ORF, open reading frame; RFLP, restriction fragment length polymorphism; T65, Taichung 65; UTR, untranslated region.

Journal Article.  6457 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.