Journal Article

RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5′ Untranslated Region in a Redox-Dependent Manner

Yanxin Shen, Avihai Danon and David A. Christopher

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 10, pages 1071-1078
Published in print October 2001 | ISSN: 0032-0781
Published online October 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce142
RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5′ Untranslated Region in a Redox-Dependent Manner

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The 5′ untranslated region (5′UTR) of the psbA mRNA (psbA encodes the PSII reaction center protein, D1) is a key site for RNA–protein interactions in the post-transcriptional regulation of gene expression. In this study, we mapped the major psbA mRNA 5′-terminus at –77 nt, and two minor termini clusters centered at –48 and –64 nt, upstream from the psbA translational start codon of Arabidopsis thaliana. RNA mobility shift, RNase protection and UV-crosslinking assays were used to characterize the interaction of chloroplast proteins with the RNA 5′UTR. RNA–protein interactions depended upon a thermolabile secondary structure and specific sequences in a 35 nt region of the 5′UTR, which were 80% conserved with the psbA 5′UTRs from five other plants. Major and minor proteins of 43- and 30-kDa, respectively, were detected by UV-crosslinking to RNA. Oxidizing conditions abolished the association of the proteins with the 5′UTR, while RNA-binding activity was recovered upon incubation with a reductant. Based on these findings, we hypothesize that post-transcriptional regulation of psbA gene expression in chloroplasts of vascular plants involves redox-dependent interactions between specific sequences in the 5′UTR and 43- and 30-kDa RNA-binding proteins.

Keywords: Key words: Arabidopsis thaliana — Chloroplast — psbA gene — Post-transcriptional — Redox-regulated — RNA binding proteins.; Abbreviations: DTNB, dithionitrobenzoic acid; RB38, RB47, RB60, RNA binding proteins 38, 47, and 60.

Journal Article.  6084 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.