Journal Article

Sugar-Induced Increase in Cytosolic Ca<sup>2+</sup> in <i>Arabidopsis thaliana</i> Whole Plants

Takuya Furuichi, Izumi C. Mori, Koji Takahashi and Shoshi Muto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 10, pages 1149-1155
Published in print October 2001 | ISSN: 0032-0781
Published online October 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce150
Sugar-Induced Increase in Cytosolic Ca2+ in
Arabidopsis thaliana Whole
Plants

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Using Ca2+-dependent photoprotein aequorin-transformed Arabidopsis thaliana, sugar-induced increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) was investigated by luminescence imaging technique. When 0.1 M sucrose was fed to roots of autotrophically grown intact whole plants whose roots had been incubated overnight with coelenterazine to reconstitute aequorin systemically, strong and transient (within 20 s) luminescence was observed in the roots; that luminescence was followed by weak luminescence moving from the lower leaves to the upper leaves. The moving rate of luminescence was roughly comparable to that of [14C]sucrose. Application of 0.1 M glucose or fructose induced transient luminescence in excised leaves. No such luminescence was observed in heterotrophically grown (with sucrose) whole plants or in excised tissues. mRNA levels of sucrose-H+ symporter genes AtSUC1 and AtSUC2 were higher in autotrophic plants than in heterotrophic plants. These results indicate that influx of transported sucrose together with H+ into the mesophyll cells of autotrophic plants may depolarize the membrane potential, and subsequently activate a voltage-gated Ca2+ channel on the plasma membrane, resulting in a [Ca2+]cyt increase. The [Ca2+]cyt increase might initiate Ca2+ signaling leading to the expression of genes related to biosynthesis of storage carbohydrates. Hexoses, when applied, might also be involved in the [Ca2+]cyt increase mediated by monosaccharide-H+ co-transporters.

Keywords: Key words: Aequorin — Arabidopsis thaliana — Calcium signaling — Luminescence imaging — Sucrose-H+ symporter — Sugar signal transduction.; Abbreviations: [Ca2+]cyt, cytosolic free calcium concentration; EGTA, ethylene glycol-bis(β-aminoethyl ether) N, N, N′,N′-tetraacetic acid; GM+Suc medium, GM medium containing 1% sucrose; GM–Suc medium, GM medium depleted sucrose; GM+Suc plant, plant grown with GM+Suc medium; GM–Suc plant, plant grown with GM–Suc medium; RT-PCR, reverse transcriptase-PCR.

Journal Article.  4442 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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