Journal Article

Identification of a Gene Required for <i>cis</i>-to-<i>trans</i> Carotene Isomerization in Carotenogenesis of the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803

Kazumori Masamoto, Hajime Wada, Takakazu Kaneko and Shinichi Takaichi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 12, pages 1398-1402
Published in print December 2001 | ISSN: 0032-0781
Published online December 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce167
Identification of a Gene Required for cis-to-trans Carotene Isomerization in Carotenogenesis of the Cyanobacterium Synechocystis sp. PCC 6803

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A disruptive mutant of the sll0033 gene of the cyanobacterium Synechocystis sp. PCC 6803 produced primarily cis carotenes and small amounts of all-trans carotenes, but no xanthophylls, under dark conditions. Under light conditions, however, it produced normal carotenoids, that were the same as those produced by wild-type cells grown under both light and dark conditions. When the mutant cells cultured under dark conditions were irradiated, cis-isomers of carotenes were converted to all-trans lycopene. These findings demonstrate that this gene, designated crtH, is involved in the isomerization of cis-carotenes to all-trans forms in dark conditions, and that cis-carotenes were also converted to all-trans forms under light conditions by photoisomerization.

Keywords: Key words: Carotene isomerase — crtH — Cyanobacteria — cis-Lycopene — Photoisomerization — Synechocystis sp. PCC 6803.; Abbreviations: LAHG, light-activated-heterotrophic-growth; PMG, photomixotrophic-growth; PAG, photoautotrophic-growth.

Journal Article.  3135 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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