Journal Article

A Pathogen-Induced Chitin-Binding Protein Gene from Pepper: Its Isolation and Differential Expression in Pepper Tissues Treated with Pathogens, Ethephon, Methyl Jasmonate or Wounding

Sung Chul Lee, Young Jin Kim and Byung Kook Hwang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 12, pages 1321-1330
Published in print December 2001 | ISSN: 0032-0781
Published online December 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce168
A Pathogen-Induced Chitin-Binding Protein Gene from Pepper:  Its Isolation and Differential Expression in Pepper Tissues Treated with Pathogens, Ethephon, Methyl Jasmonate or Wounding

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A chitin-binding protein (CBP) cDNA (CACBP1) was isolated from a cDNA library of pepper (Capsicum annuum L.) leaves infected with Xanthomonas campestris pv. vesicatoria. The deduced amino acid sequence of the CACBP1 gene which has chitin-binding domain and hinge region shares a high level of identity with CBP sequences from tomato, potato and tobacco. The CACBP1 gene was organ-specifically regulated in pepper plants, and differentially induced during the compatible and incompatible interactions of pepper with X. campestris pv. vesicatoria or Phytophthora capsici. Expression of the CACBP1 gene was rapidly induced in the incompatible interactions upon pathogen infection. Transcripts of the CACBP1 gene was highly inducible in the leaves of matured pepper plants by Colletotrichum coccodes infection. In situ hybridization results showed that CACBP1 mRNA was expressed in the phloem area of vascular bundles in C. coccodes-infected leaf tissues. The pathogen-inducible CACBP1 gene was also strongly induced and accumulated in pepper leaves by ethephon, methyl jasmonate or wounding. These data suggest that ethylene and jasmonate may act as signal molecules in the signal transduction pathways of the CBP gene induction during the pepper defense- or pathogenesis-related plant responses.

Keywords: Key words: Colletotrichum coccodes — In situ hybridization — Pepper — Phytophthora capsici — Xanthomonas campestris pv. vesicatoria.; Nucleotide sequence data have been deposited in EMBL/GenBank database under accession number AF112867.

Journal Article.  7116 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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