Journal Article

Differential Expression of Two Cytochrome P450s Involved in the Biosynthesis of Flavones and Anthocyanins in Chemo-Varietal Forms of <i>Perilla frutescens</i>

Chika Kitada, Zhizhong Gong, Yoshikazu Tanaka, Mami Yamazaki and Kazuki Saito

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 12, pages 1338-1344
Published in print December 2001 | ISSN: 0032-0781
Published online December 2001 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pce169
Differential Expression of Two Cytochrome P450s Involved in the Biosynthesis of Flavones and Anthocyanins in Chemo-Varietal Forms of Perilla frutescens

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In Perilla frutescens, there are two varietal forms of anthocyanin accumulation, i.e. red and green forms. The cDNA clones encoding flavone synthase II (FSII) and flavonoid 3′-hydroxylase (F3′H), two cytochrome P450s that are involved in the biosynthesis of flavones and anthocyanins, were isolated from P. frutescens. The FSII cDNA encoded a 57.1 kDa protein designated as CYP93B6, and the F3′H cDNA encoded 57.5 kDa protein designated as CYP75B4. Recombinant CYP93B6 expressed in yeast converted flavanones to flavones with Km values of 8.8–11.9 µM. Recombinant CYP75B4 catalyzed 3′-hydroxylation of flavanones to the corresponding compounds with Km values of 18–20 µM. The CYP93B6 transcript accumulated to an equal level in leaves of both red and green forms of P. frutescens, in agreement with the accumulation pattern of flavones in the leaves. However, the CYP75B4 transcript was predominantly expressed in the red form of P. frutescens, and its expression was induced by light in conjunction with other transcripts of biosynthetic enzymes of anthocyanin. These results indicate that gene expression of a set of anthocyanin biosynthetic enzymes including F3′H is regulated coordinately only in the red form of P. frutescens but not in the green form, whilst FSII gene expression is controlled in a similar manner in red and green forms of P. frutescens.

Keywords: Key words: Anthocyanin — Cytochrome P450 — Flavone — Flavonoid — Perilla frutescens.; Abbreviations: F2H, (2S)-flavone 2-hydroxylase; F3′H, flavonoid 3′-hydroxylase; F3′5′H, flavonoid 3′,5′-hydroxylase; FSII, flavone synthase II; HPLC, high performance liquid chromatography; ORF, open reading frame.

Journal Article.  5002 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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