Journal Article

Involvement of Superoxide Generation in Salicylic Acid-Induced Stomatal Closure in <i>Vicia faba</i>

Izumi C. Mori, Reinhard Pinontoan, Tomonori Kawano and Shoshi Muto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 12, pages 1383-1388
Published in print December 2001 | ISSN: 0032-0781
Published online December 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce176
Involvement of Superoxide Generation in Salicylic Acid-Induced Stomatal Closure in Vicia faba

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Salicylic acid (SA), the known mediator of systemic acquired resistance, induced stomatal closure of Vicia faba L. Application of SA to the epidermal peels evoked an elevation of chemiluminescence of Cripridina lucigenin-derived chemiluminescent reagent (CLA) which is sensitive to superoxide anion (O2·–). The SA-induced generation of chemiluminescence was suppressed by O2·–-specific scavengers superoxide dismutase (SOD) and 4,5-dihydroxy-1,3-benzenedisulfonic acid (Tiron). These results suggest that O2·– was generated in epidermal peels by SA-treatment. A peroxidase inhibitor salicylhydroxamic acid (SHAM) inhibited guaiacol peroxidase activity and suppressed the SA-induced CLA chemiluminescence in the epidermal peels, suggesting that O2·– generation occurred by the peroxidase-catalyzed reaction as proposed for SA-treated tobacco cell suspension culture [Kawano et al. (1998) Plant Cell Physiol. 39: 721]. SOD, Tiron or SHAM suppressed the SA-induced stomatal closure. Moreover, application of superoxide-generating system also induced stomatal closure. These results support the concept of involvement of reactive oxygen species in signal transduction in SA-induced stomatal closure.

Keywords: Key words: Peroxidase — Salicylic acid — Stomatal closure — Superoxide anion — Vicia faba.; Abbreviations: [Ca2+]cyt, cytosolic free calcium ion concentration; CLA, Cripridina lucigenin-derived chemiluminescent agent (2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrasin-3-one); DMSO, dimethylsulfoxide; DPI, diphenylene iodonium; EGTA, ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic acid; GPX, guaiacol peroxidase; O2·–, superoxide anion; rlu, relative luminescence unit; ROS, reactive oxygen species; SA, salicylic acid; SAR, systemic acquired resistance; SHAM, salicylhydroxamic acid; SOD, CuZn-superoxide dismutase.

Journal Article.  3564 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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