Journal Article

The Phytochrome A Specific Signaling Component PAT3 is a Positive Regulator of Arabidopsis Photomorphogenesis

Mathias Zeidler, Cordelia Bolle and Nam-Hai Chua

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 11, pages 1193-1200
Published in print November 2001 | ISSN: 0032-0781
Published online November 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce177
The Phytochrome A Specific Signaling Component PAT3 is a Positive Regulator of Arabidopsis Photomorphogenesis

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Phytochrome A plays a major role in early seedling development by triggering the transition from etiolated growth to greening. Seedlings germinated under constant far-red (FR) light show a partially de-etiolated phenotype that is not seen in phyA mutants. This phytochrome A specific response was used to screen a population of T-DNA mutagenized Arabidopsis seedlings. One mutant line, pat3 (phytochrome A signal transduction3), which showed no inhibition of hypocotyl elongation under FR light conditions and no FR-induced killing response, contained a T-DNA insertion in a 609-bp ORF. The recessive mutation co-segregated with the T-DNA resistance marker and could be allelic to fhy1. A 2,248-bp genomic fragment of the PAT3 locus can complement the pat3 mutant phenotype. PAT3 transcript peaked 3 d after germination and was downregulated by light. PAT3 has no significant homology to any known protein and shows no preferential cellular localization. The protein can activate transcription in yeast when fused to the GAL4 DNA-binding domain. Our results show that PAT3 is a positive regulator of phytochrome A signal transduction.

Keywords: Key words: Arabidopsis — FHY1 — Hypocotyl elongation — PAT3 — Phytochrome A — Signal transduction.; Abbreviations: B, blue; D, darkness; FR, far red; GFP, green fluorescent protein; HIR, high irradiance response; NES, nuclear export signal; NLS, nuclear localization signal; phyA, phytochrome A; POR, protochlorophyllide oxidoreductase; R, red; SSLP, simple sequence length polymorphism; UAS, upstream activator sequence; W, white; WT, wild type; RACE, rapid amplification of cDNA ends; ORF, open reading frame.

Journal Article.  6164 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.