Journal Article

Thioredoxin-Mediated Reductive Activation of a Protein Kinase for the Regulatory Phosphorylation of C4-form Phosphoenolpyruvate Carboxylase from Maize

Hidetoshi Saze, Yoshihisa Ueno, Toru Hisabori, Hiroaki Hayashi and Katsura Izui

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 12, pages 1295-1302
Published in print December 2001 | ISSN: 0032-0781
Published online December 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce182
Thioredoxin-Mediated Reductive Activation of a Protein Kinase for the Regulatory Phosphorylation of C4-form Phosphoenolpyruvate Carboxylase from Maize

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The activity of phosphoenolpyruvate carboxylase (PEPC, EC4.1.1.31) for the C4 photosynthesis is known to be regulated mainly in response to light/dark transitions through reversible phosphorylation by a specific protein kinase (PK). PEPC-PK with an Mr of 30 kDa was purified about 1.4 million-fold to homogeneity from maize leaves and characterized. The purified PEPC-PK was readily inactivated under mild oxidative conditions, but the activity could be recovered by dithiothreitol (DTT). The recovery by DTT was strongly accelerated by thioredoxin (Trx) from E. coli. Trxs of plant origin such as Trx-m from spinach chloroplast and Trx-h from rice cytoplasm were also effective. These results suggest the possibility of PEPC-PK being redox-regulated via Trx in vivo.

Keywords: Key words: Phosphoenolpyruvate carboxylase kinase — Purification — Redox-regulation — Regulatory phosphorylation — Thioredoxin — Zea mays.; Abbreviations: β-SH, 2-mercaptoethanol; DTNB, 5,5′-dithiobis-(2-nitrobenzoic acid); DTT, dithiothreitol; NEM, N-ethylmaleimide; PEPC, phosphoenolpyruvate carboxylase; PK, protein kinase; PMSF, phenylmethyl sulfonylfluoride; Trx, thioredoxin.

Journal Article.  5785 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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