Journal Article

The Possible Involvement of a Phosphate-Induced Transcription Factor Encoded by <i>Phi-2</i> Gene from Tobacco in ABA-signaling Pathways

Toshio Sano and Toshiyuki Nagata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 1, pages 12-20
Published in print January 2002 | ISSN: 0032-0781
Published online January 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf002
The Possible Involvement of a Phosphate-Induced Transcription Factor Encoded by Phi-2 Gene from Tobacco in ABA-signaling Pathways

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A novel phosphate-induced gene, phi-2, has been identified by its induction on addition of phosphate to phosphate-starved tobacco BY-2 cells. The predicted gene product of phi-2 has significant homology to a group of bZIP proteins involved in ABA-signaling pathways, and phi-2 also responded to ABA treatment. A previously isolated phosphate-induced gene, phi-1, (Sano et al. (1999) Plant Cell Physiol. 40: 1) was also responsive to ABA. Although phosphate addition induced semi-synchronous cell division in phosphate-starved tobacco BY-2 cells, ABA adversely affected cell division. Detailed examination revealed that the high levels of phosphate required to induce semi-synchronous cell division seemed to be perceived as indicators of stress by the cells. One of the stress indicators perceived by the cells is a cytoplasmic pH change, to which phi-2 and phi-1 genes respond. The different components of the cell’s response to phosphate induction are discussed.

Keywords: Key words: ABA — bZIP proteins — Cytoplasmic pH — Nicotiana tabacum BY-2 cells — Phosphate — Synchronous cell division.; Abbreviations: ABRE, ABA responsive element; bZIP protein, basic-region leucine-zipper protein; DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; IBA, isobutylic acid; MI, mitotic index.

Journal Article.  5951 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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