Journal Article

Action of Xyloglucan Hydrolase within the Native Cell Wall Architecture and Its Effect on Cell Wall Extensibility in Azuki Bean Epicotyls

Tomomi Kaku, Akira Tabuchi, Kazuyuki Wakabayashi, Seiichiro Kamisaka and Takayuki Hoson

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 1, pages 21-26
Published in print January 2002 | ISSN: 0032-0781
Published online January 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf004
Action of Xyloglucan Hydrolase within the Native Cell Wall Architecture and Its Effect on Cell Wall Extensibility in Azuki Bean Epicotyls

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Xyloglucan hydrolase (XGH) has recently been purified from the cell wall of azuki bean (Vigna angularis Ohwi et Ohashi) epicotyls as a new type of xyloglucan-degrading enzyme [Tabuchi et al. (2001) Plant Cell Physiol. 42: 154]. In the present study, the effects of XGH on the mechanical properties of the cell wall and on the level and the molecular size of xyloglucans within the native wall architecture were examined in azuki bean epicotyls. When the epidermal tissue strips from the growing regions of azuki bean epicotyls were incubated with XGH, the mechanical extensibility of the cell wall dramatically increased. XGH exogenously applied to cell wall materials (homogenates) or epidermal tissue strips decreased the amount of xyloglucans via the solubilization of the polysaccharides. Also, XGH substantially decreased the molecular mass of xyloglucans in both materials. These results indicate that XGH is capable of hydrolyzing xyloglucans within the native cell wall architecture and thereby increasing the cell wall extensibility in azuki bean epicotyls.

Keywords: Key words: Azuki bean (Vigna angularis) — Cell wall extensibility — Epicotyl — Plant growth — Xyloglucan hydrolase.; Abbreviations: EXGT, endo-xyloglucan transferase; XET, xyloglucan endotransglycosylase; XGH, xyloglucan hydrolase.

Journal Article.  4346 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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