Journal Article

GA<sub>3</sub>-Induced Expression of a New Functional AAA-ATPase (FsA1) is Correlated with the Onset of Germination in <i>Fagus sylvatica</i> L. Seeds (Beechnuts)

Oscar Lorenzo, Carlos Nicolás, Gregorio Nicolás and Dolores Rodríguez

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 1, pages 27-34
Published in print January 2002 | ISSN: 0032-0781
Published online January 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf009
GA3-Induced Expression of a New Functional AAA-ATPase (FsA1) is Correlated with the Onset of Germination in Fagus sylvatica L. Seeds (Beechnuts)

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A full-length cDNA clone, named FsA1, has been isolated from a cDNA library constructed using mRNA from Fagus sylvatica L. dormant seeds (beechnuts). This clone shows high identity with members of the AAA superfamily, for ATPases Associated with a variety of cellular Activities, encoding subunit 8 of the 26S proteasome or Tat binding proteins (TBPs). Direct biochemical evidence supporting Mg2+-dependent ATPase activity has been obtained by expressing FsA1 in Escherichia coli as histidine tag fusion protein and using the recombinant protein in the stimulation of ATP hydrolysis. Analysis of the expression of FsA1 transcripts during stratification shows an increase in the presence of gibberellic acid (GA3), a treatment that proved to be efficient in breaking dormancy and increasing germination percentages of these seeds, while the addition of paclobutrazol, a well-known GA biosynthesis inhibitor, greatly reduces the expression of the clone. A low level of expression was maintained in the stratification control in H2O, where dormancy is slowly released. These results show that this new member of the AAA-ATPase family is up-regulated by GAs and its expression correlated with the germination arise in Fagus sylvatica seeds. The possible function of this protein during the transition from dormancy to germination is discussed.

Keywords: Key words: ATPase — Beechnuts (Fagus sylvatica L.) — Dormancy/germination — Gibberellic acid — Proteasome.; The nucleotide sequence reported in this paper has been submitted to the EMBL and GenBank Nucleotide Sequences Databases under accession number AJ251819.

Journal Article.  4927 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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