Journal Article

Arabidopsis GARP Transcriptional Activators Interact with the Pro-Rich Activation Domain Shared by G-Box-Binding bZIP Factors

Hiroki Tamai, Masaki Iwabuchi and Tetsuo Meshi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 1, pages 99-107
Published in print January 2002 | ISSN: 0032-0781
Published online January 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf011
Arabidopsis GARP Transcriptional Activators Interact with the Pro-Rich Activation Domain Shared by G-Box-Binding bZIP Factors

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The Pro-rich regions, found in a subset of plant bZIP transcription factors, including G-box-binding factors (GBFs) of Arabidopsis thaliana, are thought to be deeply involved in transcriptional regulation. However, the molecular mechanisms of the Pro-rich region-mediated transcriptional regulation are still largely unknown. Here we report evidence showing that two closely related Arabidopsis proteins, designated GPRI1 and GPRI2, containing a GARP DNA-binding domain, are likely partners of one or more GBFs. The results of yeast two-hybrid assays and in vitro binding assays indicated that GPRI1 can interact with the Pro-rich regions of GBF1 and GBF3. GPRI2 interacted with the Pro-rich region of GBF1. GPRI1 and GPRI2 transactivated transcription in yeast. In GPRI1 the region responsible for this activation was mapped in the N-terminal third of the protein. Transient assays showed that in Arabidopsis cells not only the N-terminal but also the C-terminal regions of GPRI1 can function as a separable activation domain. GPRI1 and GPRI2 may function in some promoters in concert with a GBF through interaction with its Pro-rich region to enhance the transcriptional level of the corresponding genes.

Keywords: Key words: Activation domain — Arabidopsis thaliana — GARP domain — G-box-binding factor — Pro-rich region — Protein-protein interaction.; Abbreviations: bZIP, basic-region leucine-zipper; GAD, Gal4p activation domain; GBD, Gal4p DNA-binding domain; GBF, G-box-binding factor; GST, glutathione S-transferase; GUS, β-glucuronidase; ORF, open reading frame.

Journal Article.  7178 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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