Journal Article

A Pharmacological Approach to Test the Diffusible Signal Activity of Reactive Oxygen Intermediates in Elicitor-Treated Tobacco Leaves

Laurent Costet, Stephan Dorey, Bernard Fritig and Serge Kauffmann

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 1, pages 91-98
Published in print January 2002 | ISSN: 0032-0781
Published online January 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf012
A Pharmacological Approach to Test the Diffusible Signal Activity of Reactive Oxygen Intermediates in Elicitor-Treated Tobacco Leaves

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The capacity of H2O2, the most stable of the reactive oxygen species (ROI), to diffuse freely across biological membranes and to signal gene expression suggests that H2O2 could function as a short-lived second messenger diffusing from cell to cell. We tested this hypothesis in tobacco plants treated with a glycoprotein elicitor. Applied at 50 nM, it induces H2O2 accumulation and the hypersensitive response restricted to the infiltrated zone 1 tissue. Stimulation of a set of defense responses also occurs in the surrounding zone 2 tissue without diffusion of the elicitor. ROI levels in zone 1 were modulated using N-acetyl-l-cysteine (NAC) as a ROI scavenger and Rose Bengal (RB) as a ROI generator. We found that ROI appeared to act as signalling intermediates in pathways leading to salicylic acid accumulation, to PR1, PR5 and 3-hydroxy-3-methylglutarylCoA reductase expression in glycoprotein-treated zone 1 tissues. Compared to the treatment with the elicitor alone, co-infiltration of the glycoprotein and NAC increased the surface of zone 2 showing PR1 and O-methyltransferase expression. Application of RB had the opposite effect. The data suggest that, in our system, ROI did not act as a cell-to-cell diffusible signal to activate PR protein and O-methyltransferase expression in zone 2.

Keywords: Key words: Cell-to-cell signalling — Hydrogen peroxide — Nicotiana tabacum — Tobacco.; Abbreviations: DPI, diphenylene iodonium; HR, hypersensitive response; NAC, N-acetyl-l-cysteine; RB, Rose Bengal; ROI, reactive oxygen intermediates; SA, salicylic acid.

Journal Article.  7103 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.