Journal Article

A Wound-Inducible Tobacco Peroxidase Gene Expresses Preferentially in the Vascular System

Katsutomo Sasaki, Susumu Hiraga, Hiroyuki Ito, Shigemi Seo, Hirokazu Matsui and Yuko Ohashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 1, pages 108-117
Published in print January 2002 | ISSN: 0032-0781
Published online January 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf013
A Wound-Inducible Tobacco Peroxidase Gene Expresses Preferentially in the Vascular System

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A tobacco peroxidase gene tpoxN1 was reported to be expressed within 1 h after wounding in leaves [Hiraga et al. (2000a) Plant Cell Physiol. 41: 165]. We describe here further results on the wound-induced tpoxN1 expression. The quick tpoxN1 induction occurred preferentially in stems and petioles, but was negligible in leaf blades even 8 h after wounding. Induced GUS activity was also detected rapidly after wounding in the stem of transgenic tobacco plants carrying the tpoxN1 promoter::GUS fusion gene, localized mainly in the vascular systems where it was maintained this level for 14 d or more. Strong GUS activity was also found in the petiole and veinlet as well as the epidermal tissue in the stem. Treatment of known inducers for wound-responsive genes such as jasmonate, 1-aminocyclopropane-1-carboxylate, spermine, phytohormones and other stress treatments did not enhance wound-induced tpoxN1 gene expression in stems at all, but rather repressed it in some cases. Studies using metabolic inhibitors suggested that phosphorylation and dephosphorylation of proteins together with de novo protein synthesis are likely to be involved in the wound-induced tpoxN1 expression as well as some other wound-responsive genes. Thus, tpoxN1 is a unique wound-inducible and possible wound-healing gene which is rapidly expressed being maintained for a long time in veins via an unknown wound-signaling pathway(s).

Keywords: Key words: Gene expression — Signal transduction — Stem — Tobacco peroxidase — Vascular system — Wounding.; Abbreviations: ABA, abscisic acid; ACC, 1-aminocyclopropane-1-carboxylate; ACO, ACC oxidase; BA, benzyladenine; CHX, cycloheximide; EST, expressed sequence tags; GUS, β-glucuronidase; MeJA, methyl jasmonic acid; MeSA, methyl salicylic acid; NAA, naphthaleneacetic acid; Spm, spermine; PI, proteinase inhibitor; POX, peroxidase; PR, pathogenesis-related; UTR, untranslated region.

Journal Article.  7243 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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