Journal Article

Experimentally Determined Sequence Requirement of ACGT-Containing Abscisic Acid Response Element

Tsukaho Hattori, Masako Totsuka, Tokunori Hobo, Yasuaki Kagaya and Akiko Yamamoto-Toyoda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 1, pages 136-140
Published in print January 2002 | ISSN: 0032-0781
Published online January 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf014
Experimentally Determined Sequence Requirement of ACGT-Containing Abscisic Acid Response Element

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The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, Osem. This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.

Keywords: Key words: ABA — ABRE — bZIP protein — Rice (Oryza sativa) — Stress.; Abbreviations: ABREs, ABA-responsive elements; bZIP, basic region-leucine zipper; FI, fold-induction; IL, induced level; PCR, polymerase chain reaction; GUS, β-glucuronidase.

Journal Article.  3001 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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