Journal Article

Enhanced Susceptibility of Photosynthesis to Low-Temperature Photoinhibition due to Interruption of Chill-Induced Increase of S-Adenosylmethionine Decarboxylase Activity in Leaves of Spinach (<i>Spinacia oleracea</i> L.)

Lixiong He, Kazuyoshi Nada, Yoshihisa Kasukabe and Shoji Tachibana

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 2, pages 196-206
Published in print February 2002 | ISSN: 0032-0781
Published online February 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf021
Enhanced Susceptibility of Photosynthesis to Low-Temperature Photoinhibition due to Interruption of Chill-Induced Increase of S-Adenosylmethionine Decarboxylase Activity in Leaves of Spinach (Spinacia oleracea L.)

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The possible involvement of polyamines in the chilling tolerance of spinach (Spinacia oleracea L.) was investigated focusing on photosynthesis. During chilling at 8/5°C (day/night) for 6 d, S-adenosylmethionine decarboxylase (SAMDC) activity increased significantly in leaves in parallel with the increase in putrescine and spermidine (Spd) content in leaves and chloroplasts. Treatment of leaves with methylglyoxal-bis(guanylhydrazone) (MGBG), an SAMDC inhibitor, resulted in the deterioration of plant growth and photosynthesis under chilling conditions, which was reversed by the concomitant treatment with Spd through the roots. Plants treated with MGBG showed lower photochemical efficiency of PSII than either the control or plants treated with MGBG plus Spd during chilling and even after transfer to warm conditions, suggesting an increase of photoinhibition due to low Spd in chloroplasts. Indeed, MGBG-treated plants had much lower activities of thylakoid electron transport and enzymes in carbon metabolism as well as higher degrees of lipid peroxidation of thylakoid membranes compared to the control. These results indicate that the enhanced activity of SAMDC with a consequential rise of Spd in chloroplasts is crucial for the cold acclimation of the photosynthetic apparatus in spinach leaves.

Keywords: Key words: Low temperature — Photoinhibition — Photosynthesis — Polyamine — S-adenosylmethionine decarboxylase (EC 4.1.1.50) – Spinach.; Abbreviations: ADC, arginine decarboxylase; DPC, 1,5-diphenylcarbazide; FBPase, fructose-1,6-bisphosphatase; MGBG, methylglyoxal-bis(guanylhydrazone); ODC, ornithine decarboxylase; PPFD, photosynthetic photon flux density; Put, putrescine; ROS, reactive oxygen species; Spd, spermidine; Spm, spermine; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase; SAMDC, S-adenosylmethionine decarboxylase.

Journal Article.  7122 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.