Journal Article

A Gene Encoding Multidrug Resistance (MDR)-Like Protein is Induced by Aluminum and Inhibitors of Calcium Flux in Wheat

Takayuki Sasaki, Bunichi Ezaki and Hideaki Matsumoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 2, pages 177-185
Published in print February 2002 | ISSN: 0032-0781
Published online February 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf025
A Gene Encoding Multidrug Resistance (MDR)-Like Protein is Induced by Aluminum and Inhibitors of Calcium Flux in Wheat

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A cDNA clone exclusively induced by aluminum (Al) was isolated from root apices of wheat (Triticum aestivum L.) by the differential display method. The predicted amino acid sequence exhibited homology to the multidrug resistance (MDR) proteins that is known as a member of the ATP-binding cassette (ABC) protein superfamily. Thus this gene was named TaMDR1 (Triticum aestivum MDR). TaMDR1 was induced as a function of Al concentration in the range from 5 to 50 µM, which is in the range of Al content in natural acid soil environment. The concentration required for the induction was lower in the Al-sensitive cultivar than in the Al-tolerant cultivar, indicating that the accumulation of TaMDR1 mRNA was associated with the events caused by Al toxicity rather than Al tolerance. TaMDR1 was significantly induced by the exposure to lanthanum, gadolinium and ruthenium red, which are known as inhibitors of calcium channels. Furthermore, decreasing of calcium ion in growth medium caused stimulation of the gene expression. These results suggested that the induction of TaMDR1 is caused by the breaking of calcium homeostasis which occurred at early stage of Al toxicity.

Keywords: Key words: ABC protein — Aluminum stress — Calcium homeostasis — MDR — Wheat (Triticum aestivum L.).; Abbreviations: Al, aluminum; ABC protein, ATP-binding cassette protein; EGTA, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; MDR, multidrug resistance; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription-PCR.

Journal Article.  5396 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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