Journal Article

Expression of the <i>AtGH3a</i> Gene, an Arabidopsis Homologue of the Soybean <i>GH3</i> Gene, is Regulated by Phytochrome B

Shin-ichiro Tanaka, Nobuyoshi Mochizuki and Akira Nagatani

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 3, pages 281-289
Published in print March 2002 | ISSN: 0032-0781
Published online March 2002 | e-ISSN: 1471-9053 | DOI:
Expression of the AtGH3a Gene, an Arabidopsis Homologue of the Soybean GH3 Gene, is Regulated by Phytochrome B

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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Light is one of the most crucial environmental cues for plants. Phytochrome, one of the major photoreceptors of plants, regulates expression of many genes. We screened for Arabidopsis promoter trap lines that exhibited light-dependent reporter gene expression. Molecular analysis of one such line revealed that a reporter gene was inserted near an Arabidopsis homologue of the soybean GH3 gene, AtGH3a. We performed RNA gel blot analysis to further characterize the response of the AtGH3a gene to light. In response to the end-of-day far-red light treatment, the expression increased substantially. Analysis of the phyB-deficient mutant indicated that this light response is under the control of phytochrome B. The expression was also induced by exogenous auxin. Furthermore, the light response was substantially reduced in the auxin-related axr2 mutant. Taken together, it is suggested that phytochrome B regulates the expression of genes by altering the levels of auxin.

Keywords: Key words: Auxin — End-of-day far-red light — Gene expression — Phytochrome B — Promoter trap lines — Shade avoidance.; Abbreviations: 2,4-D; 2,4-dichlorophenoxyacetic acid; FR, far-red light; GUS, β-glucuronidase; 4-MU, 4-methylumberyferon; phyA, phytochrome A; phyB, phytochrome B; R, red light; T-DNA, transfer DNA; X-Gluc, 5-bromo-4-chloro-3-indolyl β-d-glucuronide.

Journal Article.  7393 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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