Journal Article

Diurnal Change of Cucumber CPD Photolyase Gene (<i>CsPHR</i>) Expression and Its Physiological Role in Growth under UV-B Irradiation

Shinya Takahashi, Nobuyoshi Nakajima, Hikaru Saji and Noriaki Kondo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 3, pages 342-349
Published in print March 2002 | ISSN: 0032-0781
Published online March 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf038
Diurnal Change of Cucumber CPD Photolyase Gene (CsPHR) Expression and Its Physiological Role in Growth under UV-B Irradiation

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Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves. The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase. Transformation with the cDNA restored the impaired photorepair activity of an Escherichia coli mutant, indicating that this cDNA encodes a functional cucumber CPD photolyase (CsPHR). The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09 : 00 and 12 : 00, respectively. Supplemental UV-B irradiation in the middle of the light period had little effect on the growth of the first leaves, while the supplemental irradiation in the early morning or late afternoon strongly retarded the leaf growth. These results suggest that the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation.

Keywords: Key words: Cucumis sativus — Cyclobutane pyrimidine dimer —Diurnal change — DNA photolyase (EC 4.1.99.3) — Growth retardation —Photorepair.; Abbreviations: CPD, cyclobutane pyrimidine dimer; DTT, dithiothreitol; ELISA, enzyme-linked immunosorbent assay; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription–polymerase chain reaction.

Journal Article.  6213 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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