Journal Article

Identification and Characterization of a Gene Encoding Drought-Inducible Protein Localizing in the Bundle Sheath Cell of Sugarcane

Bambang Sugiharto, Netty Ermawati, Hitoshi Mori, Koh Aoki, Keiko Yonekura-Sakakibara, Tomoyuki Yamaya, Tatsuo Sugiyama and Hitoshi Sakakibara

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 3, pages 350-354
Published in print March 2002 | ISSN: 0032-0781
Published online March 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf039
Identification and Characterization of a Gene Encoding Drought-Inducible Protein Localizing in the Bundle Sheath Cell of Sugarcane

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  • Biochemistry
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We have identified a drought-inducible gene, designated as SoDip22, in sugarcane leaves. The cDNA encoded a hydrophilic protein with a calculated molecular mass of 15.9 kDa and the amino acid sequence was similar to that of ABA, stress and ripening-inducible protein from various plant species. ABA or mannitol-treatment of the detached leaves also induced SoDip22 expression. Stepwise homogenization of the stressed leaves showed that SoDip22 is localized in bundle sheath cells. These results suggest that SoDip22 functions to adapt to drought stress in the bundle sheath cell, and that the signaling pathway for the induction is, at least in a part, mediated by ABA.

Keywords: Key words: ABA — Asr proteins — C4 plant — Drought stress — Saccharum officinarum — Sugarcane.; Abbreviations: Asr, ABA stress and ripening-inducible; BSC, bundle sheath cell; MC, mesophyll cell; PEG, polyethyleneglycol; PEPC, phosphoenolpyruvate carboxylase; PVDF, polyvinylidene difluoride; RACE, rapid amplification of cDNA ends; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase; RT-PCR, reverse transcription-PCR.

Journal Article.  2763 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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