Journal Article

Identification and Characterization of Two Phage-Type RNA Polymerase cDNAs in the Moss <i>Physcomitrella patens</i>: Implication of Recent Evolution of Nuclear-Encoded RNA Polymerase of Plastids in Plants

Yukihiro Kabeya, Kazuyoshi Hashimoto and Naoki Sato

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 3, pages 245-255
Published in print March 2002 | ISSN: 0032-0781
Published online March 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf041
Identification and Characterization of Two Phage-Type RNA Polymerase cDNAs in the Moss Physcomitrella patens: Implication of Recent Evolution of Nuclear-Encoded RNA Polymerase of Plastids in Plants

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We isolated two cDNAs for the genes PpRPOT1 and PpRPOT2 that encode phage-type RNA polymerases (RPOTs) from Physcomitrella patens. Transcriptional activity of the encoded proteins was demonstrated by an in vitro transcription assay. Transiently expressed RPOT green fluorescent protein fusion proteins were both targeted to mitochondria. These results suggest that both PpRPOT1 and PpRPOT2 proteins function as mitochondrial RNA polymerases. Detailed phylogenetic analysis using neighbor-joining and maximum-likelihood methods with both DNA and protein sequences indicated that the two genes of P. patens form a sister group to all flowering plant genes. This suggests that the gene duplication leading to the production of plastid-type isozymes occurred after the separation of vascular plant lineage from bryophyte lineage. We therefore suggest that the generation of nuclear-encoded RNA polymerase of chloroplast is a rather recent event during the evolution of land plants.

Keywords: Key words: Mitochondrion —Molecular phylogeny — Nuclear encoded RNA polymerase — Phage-type RNA polymerase — Physcomitrella patens — Plastid.; Abbreviations: GFP, green fluorescent protein; ML, maximum-likelihood; NCII promoter, non-consensus II promoter; NEP, nuclear-encoded RNA polymerase; Ni-NTA, nickel nitrilotriacetic acid; NJ, neighbor-joining; PEP, plastid-encoded RNA polymerase; RNAP, RNA polymerase; RPOT, phage-type RNA polymerase.

Journal Article.  6096 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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