Journal Article

Effects of Light and Low Oxygen Tension on Pigment Biosynthesis in <i>Halobacterium salinarum</i>, Revealed by a Novel Method to Quantify Both Retinal and Carotenoids

Wael S. M. El-Sayed, Shinichi Takaichi, Haruo Saida, Masahiro Kamekura, Mohamed Abu-Shady, Humitake Seki and Tomohiko Kuwabara

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 4, pages 379-383
Published in print April 2002 | ISSN: 0032-0781
Published online April 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf044
Effects of Light and Low Oxygen Tension on Pigment Biosynthesis in Halobacterium salinarum, Revealed by a Novel Method to Quantify Both Retinal and Carotenoids

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A novel method for analyzing halobacterial pigments was developed, in which retinal was liberated from halobacterial rhodopsins as retinal oxime by hydroxylamine, ethyl β-apo-8′-carotenoate was introduced as an internal standard, and the pigments including bacterioruberin and β-carotene were analyzed by HPLC at the same time. With this method, we revealed that light enhances the biosynthesis of bacterioruberin and the conversion of β-carotene to retinal, but does not affect β-carotene biosynthesis in Halobacterium salinarum strain Oyon Moussa-16. Low oxygen tension given in the light brought a slight increase in retinal accumulation, although its biosynthesis from β-carotene is an oxygenation reaction. This paradox could be explained by the increase in β-carotene biosynthesis.

Keywords: Key words: Bacterioruberin — Hydroxylamine — Low oxygen tension — Retinal oxime — Total β-carotene synthesis.; Abbreviations: loc, low organic components; TBS, total β-carotene synthesis.

Journal Article.  3542 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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