Journal Article

Visualization of Peroxisomes in Living Plant Cells Reveals Acto-Myosin-Dependent Cytoplasmic Streaming and Peroxisome Budding

Gregory Jedd and Nam-Hai Chua

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 4, pages 384-392
Published in print April 2002 | ISSN: 0032-0781
Published online April 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf045
Visualization of Peroxisomes in Living Plant Cells Reveals Acto-Myosin-Dependent Cytoplasmic Streaming and Peroxisome Budding

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Here we examine peroxisomes in living plant cells using transgenic Arabidopsis thaliana plants expressing the green fluorescent protein (GFP) fused to the peroxisome targeting signal 1 (PTS1). Using time-lapse laser scanning confocal microscopy we find that plant peroxisomes exhibit fast directional movement with peak velocities approaching 10 µm s–1. Unlike mammalian peroxisomes which move on microtubules, plant peroxisome movement is dependent on actin microfilaments and myosin motors, since it is blocked by treatment with latrunculin B and butanedione monoxime, respectively. In contrast, microtubule-disrupting drugs have no effect on peroxisome streaming. Peroxisomes were further shown to associate with the actin cytoskeleton by the simultaneous visualization of actin filaments and peroxisomes in living cells using GFP-talin and GFP-PTS1 fusion proteins, respectively. In addition, peroxisome budding was observed, suggesting a possible mechanism of plant peroxisome proliferation. The strong signal associated with the GFP-PTS1 marker also allowed us to survey cytoplasmic streaming in different cell types. Peroxisome movement is most intense in elongated cells and those involved in long distance transport, suggesting that higher plants use cytoplasmic streaming to help transport vesicles and organelles over long distances.

Keywords: Key words: Actin — Cytoplasmic streaming — GFP — Myosin — Peroxisome — Phloem.; Abbreviations: GFP, green fluorescent protein; ER, endoplasmic reticulum; PTS1, peroxisome targeting signal-1.

Journal Article.  5451 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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