Journal Article

Regulation of Gene Expression by Low Levels of Ultraviolet-B Radiation in <i>Pisum sativum</i>: Isolation of Novel Genes by Suppression Subtractive Hybridisation

Helena Sävenstrand, Mikael Brosché and Åke Strid

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 4, pages 402-410
Published in print April 2002 | ISSN: 0032-0781
Published online April 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf047
Regulation of Gene Expression by Low Levels of Ultraviolet-B Radiation in Pisum sativum: Isolation of Novel Genes by Suppression Subtractive Hybridisation

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Suppression subtractive hybridisation was used to isolate genes differentially regulated by low levels (UV-BBE,300 0.13 W m–2) of ultraviolet-B radiation (UV-B; 290–320 nm) in Pisum sativum. Six genes were regulated, two of which were novel. The mRNA levels for these two (PsTSDC and PsUOS1) were increased and depressed by UV-B treatment, respectively. Domains in the PsTSDC translation product was similar to TIR (Toll-Interleukin-1 receptor-similar) domains and a NB-ARC domain (nucleotide-binding domain in APAF-1, R gene products and CED-4). The PsUOS1 translation product was similar to an open reading frame in Arabidopsis. Genes encoding embryo-abundant protein (PsEMB) and S-adenosyl-l-methionine synthase (PsSAMS) were induced by UV-B, whereas the transcript levels for genes encoding sucrose transport protein (PsSUT) or ribulose-5-phosphate 3-epimerase (PsR5P3E) were decreased. These regulation patterns are novel, and the PsEMB and PsR5P3E sequences are reported for the first time. The stress-specificity of regulation of these genes were tested by ozone fumigation (100 ppb O3). Qualitatively, the similarity of expression after both UV-B and ozone exposure suggests that, for these genes, similar stress-response pathways are in action.

Keywords: Key words: Gene expression — Ozone — Pisum sativum — Suppression subtractive hybridisation — UV-B radiation.; Abbreviations: EMB, embryo-abundant protein; EST, expressed sequence tags; NB-ARC, nucleotide-binding domain shared by APAF-1, certain R gene products and CED-4; R5P3E, ribulose-5-phosphate-3-epimerase; SAM, S-adenosyl-l-methionine; SAMS, S-adenosyl-l-methionine synthase; SSH, suppression subtractive hybridisation; SUT, sucrose transport protein; TIR, toll-interleukin-1 receptor-similar domain; TSDC, TIR-similar-domain containing protein; UOS1, UV-B and ozone similarly-regulated protein 1; UV-A, ultraviolet-A radiation; UV-B, ultraviolet-B radiation; UV-BBE,300, biologically effective ultraviolet-B radiation normalised to 300 nm.

Journal Article.  6578 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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