Journal Article

Cell Wall Invertase in Developing Rice Caryopsis: Molecular Cloning of <i>OsCIN1</i> and Analysis of its Expression in Relation to its Role in Grain Filling

Tatsuro Hirose, Makoto Takano and Tomio Terao

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 4, pages 452-459
Published in print April 2002 | ISSN: 0032-0781
Published online April 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf055
Cell Wall Invertase in Developing Rice Caryopsis: Molecular Cloning of OsCIN1 and Analysis of its Expression in Relation to its Role in Grain Filling

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  • Biochemistry
  • Molecular and Cell Biology
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To establish the significance of cell wall invertase in grain filling of rice (Oryza sativa L.), we cloned a cDNA for a cell wall invertase from developing grains of rice. The cDNA, designated OsCIN1, contains an open reading frame of 1731 bp encoding a polypeptide of 577 amino acid residues. The deduced amino acid sequence showed typical features of the cell wall invertases, including a β-fructosidase motif and a cysteine catalytic site, and shared 78.6 and 73.7% identity with maize cell wall invertases, Incw1 and Incw2, respectively. OsCIN1 is expressed in roots, in sink- and source-leaves, and in panicles. During the course of grain filling in the caryopses, OsCIN1 transcript is detectable only in the very early stage of their development, 1–4 d after flowering, when the cell wall invertase activity is the highest and the increase in caryopsis length is rapid. In situ localization of the mRNA revealed that OsCIN1 is expressed preferentially in the vascular parenchyma of the dorsal vein, integument and its surrounding cells, and is expressed weakly in the nucellar projection and nucellar epidermis. These results suggest that, during the early stage of caryopsis development, OsCIN1 is important for supplying a carbon source to developing filial tissues by cleaving unloaded sucrose in the apoplast.

Keywords: Key words: Cell wall invertase (EC 3.2.1.26) — Grain filling — Oryza sativa L. — Sucrose transport.; Abbreviations: CWI, cell wall invertase; DAF, days after flowering; DIG, digoxigenin; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription-PCR.

Journal Article.  5620 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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