Journal Article

Isolation of Rice Genes Possibly Involved in the Photoperiodic Control of Flowering by a Fluorescent Differential Display Method

Ryousuke Hayama, Takeshi Izawa and Ko Shimamoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 5, pages 494-504
Published in print May 2002 | ISSN: 0032-0781
Published online May 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf059
Isolation of Rice Genes Possibly Involved in the Photoperiodic Control of Flowering by a Fluorescent Differential Display Method

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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To better understand the molecular mechanisms of the photoperiodic regulation of rice, a short-day plant, we isolated 27 cDNAs that were differentially expressed in the photoperiod-insensitive se5 mutant from approximately 8,400 independent mRNA species by the use of a fluorescent differential display (FDD). For this screening, we isolated mRNAs at five different time points during the night and compared their expression patterns between se5 and the wild type. Of 27 cDNAs isolated, 12 showed diurnal expression patterns often associated with genes involved in the determination of the flowering time. In se5, expression of nine cDNAs was increased. Five of these cDNAs were up-regulated under SD, suggesting that they may promote flowering under SD. They included genes encoding a cDNA containing a putative NAC domain, the fructose-bisphosphate aldolase, and a protease inhibitor. Expression of three cDNAs was decreased in se5 but not photoperiodically regulated. These cDNAs included a rice homolog of Arabidopsis GIGANTEA (GI), lir1, and a gene for myo-inositol 1-phosphate synthase, all of which were previously shown to be under the control of circadian clocks. The expression patterns of the rice homolog of GI, OsGI, were similar to those of the Arabidopsis GI, suggesting the conservation of some mechanisms for the photoperiodic regulation of flowering between these two species.

Keywords: Key words: Circadian clock — FDD — Flowering time — Rice.; Abbreviations: GI, GIGANTEA; FDD, fluorescent differential display; LD, long day; SD, short day.

Journal Article.  7045 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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