Journal Article

<i>pilG</i> Gene Cluster and Split <i>pilL</i> Genes Involved in Pilus Biogenesis, Motility and Genetic Transformation in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803

Shizue Yoshihara, Xiaoxing Geng and Masahiko Ikeuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 5, pages 513-521
Published in print May 2002 | ISSN: 0032-0781
Published online May 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf061
pilG Gene Cluster and Split pilL Genes Involved in Pilus Biogenesis, Motility and Genetic Transformation in the Cyanobacterium Synechocystis sp. PCC 6803

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  • Molecular and Cell Biology
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The unicellular motile cyanobacterium Synechocystis sp. PCC 6803 exhibits phototactic motility that depends on the type IV-like thick pilus structure. By gene disruption analysis, we showed that a gene cluster of slr1041, slr1042, slr1043 and slr1044, whose predicted products are homologous to PatA, CheY, CheW and MCP, respectively, was more or less required for pilus assembly, motility and natural transformation competency with extraneous DNA. By sequence homology, the missing cheA-like gene in this cluster was identified as novel split genes, slr0073 and slr0322, at separate loci on the genome. This was confirmed by non-motile phenotype of their disruptants. Unique hyperpiliation was observed in the slr1042 and slr0073 disruptants, suggestive of their specific interaction with pilT1. The genes, thus identified as pil genes in this study, were designated pilG (slr1041), pilH (slr1042), pilI (slr1043), pilJ (slr1044), pilL-N (slr0073) and pilL-C (slr0322).

Keywords: Key words: Cyanobacterium — Motility — Pilus biogenesis — Split cheA — Synechocystis sp. PCC 6803 — Transformation competency.; Abbreviation: ORF, open reading frame.

Journal Article.  4238 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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