Journal Article

Tissue-Specific, Light-Regulated and Plastid-Regulated Expression of the Single-Copy Nuclear Gene Encoding the Chloroplast Rieske FeS Protein of <i>Arabidopsis thaliana</i>

Julie S. Knight, Catherine M. Duckett, James A. Sullivan, Amanda R. Walker and John C. Gray

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 5, pages 522-531
Published in print May 2002 | ISSN: 0032-0781
Published online May 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf062
Tissue-Specific, Light-Regulated and Plastid-Regulated Expression of the Single-Copy Nuclear Gene Encoding the Chloroplast Rieske FeS Protein of Arabidopsis thaliana

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The single-copy PetC gene encoding the chloroplast Rieske FeS protein of Arabidopsis thaliana consists of five exons interrupted by four introns and encodes a protein of 229 amino acid residues with extensive sequence similarity to the chloroplast Rieske proteins of other higher plants. The N-terminal 50 amino acid residues constitute a presequence for targeting to the chloroplast and the remaining 179 amino acid residues make up the mature protein. Three of the introns are in identical positions in the PetC gene of Chlamydomonas reinhardtii, suggesting that they are of ancient origin. RNA-blot hybridisation showed that the gene was expressed in shoots, but not roots, and was light regulated and repressed by sucrose. The expression of chimeric genes consisting of PetC promoter fragments fused to the β-glucuronidase (GUS) reporter gene was examined in A. thaliana and tobacco. In A. thaliana, GUS activity was detected in leaves, stems, flowers and siliques, but not in roots, and showed a strong correlation with the presence of chloroplasts. In transgenic tobacco, low levels of GUS activity were also detected in light-exposed roots. GUS activity in transgenic tobacco seedlings was light regulated and was decreased by norflurazon in the light suggesting regulation of PetC expression by plastid signals.

Keywords: Key words: Arabidopsis — Chloroplast — Light-regulated expression — Plastid signal — Rieske FeS protein.; Abbreviations: GUS, β-glucuronidase; MMLV, Moloney murine leukaemia virus; MUG, methylumbelliferyl-β-glucuronide; X-Gluc, 5-bromo-4-chloro-3-indolyl-β-glucuronide.

Journal Article.  7360 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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