Journal Article

Subcellular Localization of 1-Aminocyclopropane-1-Carboxylic Acid Oxidase in Apple Fruit

Mei-Chu Chung, Shu-Jen Chou, Lin-Yun Kuang, Yee-yung Charng and Shang Fa Yang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 5, pages 549-554
Published in print May 2002 | ISSN: 0032-0781
Published online May 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf067
Subcellular Localization of 1-Aminocyclopropane-1-Carboxylic Acid Oxidase in Apple Fruit

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  • Biochemistry
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  • Plant Sciences and Forestry

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1-Aminocyclopropane-1-carboxylic acid (ACC) oxidase catalyzes the oxidation of ACC to the gaseous plant hormone, ethylene. Although the enzyme does not contain a typical N-terminal consensus sequence for the transportation across the endoplasmic reticulum (ER), it has recently been shown to locate extracellularly by immunolocalization study. It was of interest to examine whether the enzyme contains a signal peptide that is overlooked by structure prediction. We observed that the in vitro translated apple ACC oxidase was not co-processed or imported by the canine pancreatic rough microsomes, a system widely used to identify signal peptide for protein translocation across ER, suggesting that apple ACC oxidase does not contain a signal peptide for ER transport. A highly specific polyclonal antibody raised against the recombinant apple ACC oxidase was used to examine the subcellular localization of the enzyme in apple fruit (Malus domestica, var. Golden Delicious). The location of ACC oxidase appeared to be mainly in the cytosol of the apple fruit pericarp tissue as was demonstrated by electron microscopy using immunogold-labeled antibodies. The pre-immune serum or pre-climacteric fruit control gave essentially no positive signal. Based on these observations, we conclude that ACC oxidase is a cytosolic protein.

Keywords: Key words: ACC oxidase — Ethylene — Immunocytolocalization — Malus domestica — Signal sequence.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylic acid; ACO, ACC oxidase; ER, endoplasmic reticulum; CPMM, canine pancreatic rough microsomal membranes; PVPP, polyvinylpolypyrrolidone.

Journal Article.  3835 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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