Journal Article

Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells

Yuki Yanagawa, Seiichiro Hasezawa, Fumi Kumagai, Masayoshi Oka, Masahiro Fujimuro, Tae Naito, Taro Makino, Hideyoshi Yokosawa, Keiji Tanaka, Atsushi Komamine, Junji Hashimoto, Takahide Sato and Hiroki Nakagawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 6, pages 604-613
Published in print June 2002 | ISSN: 0032-0781
Published online June 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf072
Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells

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The 26S proteasome is known to play pivotal roles in cell-cycle progression in various eukaryotic cells; however, little is known about its role in higher plants. Here we report that the subcellular distribution of the 26S proteasome is dynamically changed in a cell-cycle dependent manner in tobacco BY-2 cells as determined by immunostaining with anti-Rpn10 (a regulatory PA700 subunit) and anti-20S catalytic proteasome antibodies. The 26S proteasome was found to localize not only in nuclear envelopes and mitotic spindles but also in preprophase bands (PPBs) and phragmoplasts appearing in G2 and M phases, respectively. MG132, a proteasome inhibitor, exclusively caused cell-cycle arrest not only at the metaphase but also the early stage of PPB formation at the G2 phase and the collapse of the phragmoplast, which seems to be closely related to proteasome distribution in the cells.

Keywords: Keywords: Cell cycle — Microtubule — Proteasome — Rpn10 — Subcellular distribution — Tobacco BY-2 cells.; Abbreviations: MTs, microtubules; PPB, preprophase band.

Journal Article.  7179 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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