Journal Article

TMBP200, a Microtubule Bundling Polypeptide Isolated from Telophase Tobacco BY-2 Cells is a MOR1 Homologue

Hiroki Yasuhara, Masaaki Muraoka, Hiroki Shogaki, Hitoshi Mori and Seiji Sonobe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 6, pages 595-603
Published in print June 2002 | ISSN: 0032-0781
Published online June 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf074
TMBP200, a Microtubule Bundling Polypeptide Isolated from Telophase Tobacco BY-2 Cells is a MOR1 Homologue

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Bundles of microtubules and cross-bridges between microtubules in the bundles have been observed in phragmoplasts, but proteins responsible for forming the cross-bridges have not been identified. We isolated TMBP200, a novel microtubule bundling polypeptide with an estimated relative molecular mass of about 200,000 from telophase tobacco BY-2 cells. Ultrastructural observation of microtubules bundled by purified TMBP200 in vitro revealed that TMBP200 forms cross-bridges between microtubules. The structure of the bundles and lengths of the cross-bridges were quite similar to those observed in phragmoplasts, suggesting that TMBP200 participates in the formation of microtubule bundles in phragmoplasts. The cDNA encoding TMBP200 was cloned and the deduced amino acid sequence showed homology to a class of microtubule-associated proteins including Xenopus XMAP215, human TOGp and Arabidopsis MOR1.

Keywords: Keywords: Cross-bridge (microtubules) — Microtubule-associated proteins (MAPs) — Phragmoplast — Cytokinesis — XMAP215 — Tobacco BY-2 cells.; Abbreviations: DTT, dithiothreitol; EGTA, ethyleneglycol bis (2-aminoethylether) tetraacetic acid; PIPES, piperazine-N,N′-bis (2-ethanesulfonic acid); PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene fluoride; RACE, rapid amplification of cDNA ends.

Journal Article.  5326 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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