Journal Article

<i>OSTF1</i>: A HD-GL2 Family Homeobox Gene is Developmentally Regulated During Early Embryogenesis in Rice

Jun-Yi Yang, Mei-Chu Chung, Ching-Yu Tu and Wei-Ming Leu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 6, pages 628-638
Published in print June 2002 | ISSN: 0032-0781
Published online June 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf076
OSTF1: A HD-GL2 Family Homeobox Gene is Developmentally Regulated During Early Embryogenesis in Rice

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In many eukaryotic organisms, homeobox genes are important regulators that specify the cell fate and body plan in early embryogenesis. In this study, a gene designated OSTF1 (Oryza sativa transcription factor 1) encoding a homeodomain protein in rice was isolated and characterized. The encoded OSTF1, although sharing only approximately 51% sequence identity with other HD-GL2 members, contains four characteristic motifs (an N-terminal acidic region, a homeodomain, a truncated leucine zipper, and a START domain). OSTF1 was detected as a single copy gene in rice. The transcripts were absent in young panicle or mature spikelet before anthesis, but appeared very early in the pollinated grain with a transient profile. In vegetative tissues examined, expression was only detectable in root. In situ hybridization analysis on developing grains revealed that OSTF1 was strongly and uniformly expressed in the embryo at the globular stage and preferentially localized to the protoderm at 3–6 d after pollination. Expression was also detectable in the integument and throughout the endosperm. Although OSTF1 is not closely related to the remaining HD-GL2 members in sequences, this gene exhibits an analogous epidermis-preferential expression pattern.

Keywords: Keywords: Embryogenesis — HD-GL2 — Homeobox — In situ hybridization — Rice.; Abbreviations: BR, brassinosteroid; DAP, days after pollination; EST, expressed sequence tag; HD, homeodomain; RACE, rapid amplification of cDNA ends; RT, reverse transcription; StAR, steroidogenic acute regulatory protein; START domain, StAR-related lipid transfer domain.

Journal Article.  6540 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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