Journal Article

BP-80 and Homologs are Concentrated on Post-Golgi, Probable Lytic Prevacuolar Compartments

Yu-Bing Li, Sally W. Rogers, Yu Chung Tse, Sze Wan Lo, Samuel S. M. Sun, Guang-Yuh Jauh and Liwen Jiang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 7, pages 726-742
Published in print July 2002 | ISSN: 0032-0781
Published online July 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf085
BP-80 and Homologs are Concentrated on Post-Golgi, Probable Lytic Prevacuolar Compartments

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Prevacuolar compartments (PVCs) are membrane-bound organelles that mediate protein traffic between Golgi and vacuoles in the plant secretory pathway. Here we identify and define organelles as the lytic prevacuolar compartments in pea and tobacco cells using confocal immunofluorescence. We use five different antibodies specific for a vacuolar sorting receptor (VSR) BP-80 and its homologs to detect the location of VSR proteins. In addition, we use well-established Golgi-markers to identify Golgi organelles. We further compare VSR-labeled organelles to Golgi organelles so that the relative proportion of VSR proteins in Golgi vs. PVCs can be quantitated. More than 90% of the BP-80-marked organelles are separate from Golgi organelles; thus, BP-80 and its homologs are predominantly concentrated on the lytic PVCs. Additionally, organelles marked by anti-AtPep12p (AtSYP21p) and anti-AtELP antibodies are also largely separate from Golgi apparatus, whereas VSR and AtPep12p (AtSYP21p) were largely colocalized. We have thus demonstrated in plant cells that VSR proteins are predominantly present in the lytic PVCs and have provided additional markers for defining plant PVCs using confocal immunofluorescence. Additionally, our approach will provide a rapid comparison between markers to quantitate protein distribution among various organelles.

Keywords: Keywords: Prevacuolar compartments — Vacuolar sorting receptor.; Abbreviations: DIP, dark intrinsic protein; Man1, mannosidase I; PAC, precursor-accumulating vesicle; MVBs, multivesicular bodies; RMR protein, receptor homology region-transmembrane domain-Ring H2 motif; PSV, protein storage vacuole; PVCs, prevacuolar compartments; V-PPase, vacuolar pyrophosphatase; VSR, vacuolar sorting receptor.

Journal Article.  11863 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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