Journal Article

Ectopic Expression of an <i>Arabidopsis</i> Single Zinc Finger Gene in Tobacco Results in Dwarf Plants

Randy Dinkins, Christina Pflipsen, Allison Thompson and Glenn B. Collins

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 7, pages 743-750
Published in print July 2002 | ISSN: 0032-0781
Published online July 2002 | e-ISSN: 1471-9053 | DOI:
Ectopic Expression of an Arabidopsis Single Zinc Finger Gene in Tobacco Results in Dwarf Plants

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Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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A survey of the Arabidopsis thaliana databases revealed that single C2H2 zinc finger protein genes comprise a large gene family (~30 genes). No known phenotype has been associated with any of these genes except SUPERMAN. One of these genes, designated AtZFP10 (A. thaliana single zinc finger protein), was isolated by RT-PCR in the present study. The AtZFP10 gene was expressed at low levels in the flowers, axillary meristems and siliques, and at very low levels in the stems in Arabidopsis. Overexpression of the AtZFP10 gene driven by a constitutive promoter resulted in abnormal Arabidopsis plants and only one plant was recovered. Tobacco plants overexpressing the AtZFP10 gene displayed dwarfing, abnormal leaf phenotypes and early flowering that correlated with the level of expression of the AtZFP10 gene. No differences were observed in cell size between the AtZFP10 transgenic plants and the wild-type plants. Application of exogenous GA3 did not restore the wild-type phenotype, but it did reduce the dwarfing phenotype. Deletion of the leucine-rich region at the carboxyl terminus of the AtZFP10 gene resulted in transgenic plants that were not phenotypically different from wild-type plants suggesting a role for the leucine-rich region as essential for normal function.

Keywords: Keywords: Arabidopsis thaliana — Nicotiana tabacum — SUPERMAN — Zinc finger protein.

Journal Article.  4212 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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